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If building v2 sample sheets for instruments in addition to the ones stated, verify the ultimate sample sheet has the i5 index in the correct orientation as explained above.
Index 1: the i7 index column is within the ahead orientation (match the orientation In the event the i7 indexes within the sample sheet).
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Consult with Index 2 (i5) Orientation for more information regarding how to enter i5 bases to the sample sheet in ahead or reverse complement orientation.
A quick, integrated workflow for making ready libraries for use in a variety of sequencing programs.
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The software program mechanically creates the reverse complement as desired for Evaluation. If your sequence is reverse complemented, this may only be demonstrated during the SampleSheetUsed.csv file.
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The sequences exhibited are what's entered into the final sample sheet. click here Package definitions contain the i5 in the forward orientation. The i5 sequences will likely be properly reverse complemented and shown while in the IEM interface based upon instrument/reagent style.